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Animal physiology hill pdf download4/21/2023 We identified two genomic regions based on recombination events which had significant increases of 0.65 and 0.72% in seed protein content without a significant decrease in seed oil content. Single marker analysis and linkage analysis using 50 RHD-NILs validated the chromosome 14 protein QTL, and whole genome sequencing of RHD-NILs allowed us to reduce the QTL interval from ∼16.5 to ∼4.6 Mbp. soja: one for a novel seed oil QTL on chromosome 8 and another for a novel protein QTL on chromosome 14. We analyzed 3,015 single F 4:9 soybean plants to develop two residual heterozygotes derived near isogenic lines (RHD-NIL) populations by targeting nine SNP markers from genotype-by-sequencing, which corresponded to two novel quantitative trait loci (QTL) derived from G. Linkage mapping identified a total of seven significant quantitative trait loci on chromosomes 14 and 20 for seed protein and on chromosome 8 for seed oil with LOD scores ranging from 5.3 to 31.7 for seed protein content and from 9.8 to 25.9 for seed oil content. The objectives of our study were to examine the genetic architecture responsible for seed protein and oil using a recombinant inbred line (RIL) population derived from hybridizing a G. In both public and private soybean breeding programs, the introgression of wild soybean ( Glycine soja Siebold and Zucc.) alleles is a viable option to increase genetic diversity and identify new sources for traits of value. Modern soybean cultivars have low overall genetic variation due to repeated bottleneck events that arose during domestication and from selection strategies typical of many soybean breeding programs. 5Soybean Genomics and Improvement Laboratory, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD, United States. Bond Life Science Center, University of Missouri, Columbia, MO, United States 4Department of Health Management and Informatics, MU Institute of Data Science and Informatics and Christopher S.3Department of Electrical Engineering and Computer Science, University of Missouri, Columbia, MO, United States.2Plant Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, Columbia, MO, United States.1Division of Plant Science and Technology, University of Missouri, Columbia, MO, United States.Gillman 2, Zhen Lyu 3, Trupti Joshi 4, Mariola Usovsky 1, Qijian Song 5 and Andrew Scaboo 1*
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